Molecular Biology Core Laboratory

Director: Ranganna, Kasturi, Ph.D.

People:

• Director: Ranganna, Kasturi, Ph.D.
  

Member: Matthew, Omana
Role: Research Associate, College of Pharmacy and Health Sciences, Department of Pharmaceutical Sciences

Resources:

Instruments

• Cytomics FC 500 Flow Cytometry System ( Flow cytometer )
  

• FX Phospho Multipurpose Imaging System ( Multi-imager )
  

• Millipore MilliQ water purification system ( Water purification system )
  

• Nikon Eclipse TS100 fluorescence & Phase contrast microscope ( Inverted microscope )
  

Services

• Analysis of gene function by RNA interference ( Material analysis service )
  

  Use siRNA or shRNA plasmids both in cell culture model and animal models

• Chromatin immunoprecipitation (ChIP) assay ( Material analysis service )
  

  Analysis of protein-DNA interactions, specifically at target promoter sites under native conditions

• Conventional and real time PCR assays ( Material analysis service )
  

  Analysis of gene expression & function and miRNA analysis

• Epigenetic analysis ( Material analysis service )
  

  Significance of acetylation and methylation of histones in the regulation of gene expression: role in normal development and in disease pathogenesis

• Flow cytometry ( Material analysis service )
  

  Cell proliferation, apoptosis, altered redox state, and protein expression analysis.

• MicroRNA profiling ( Material analysis service )
  

  Studies in the context of disease pathogenesis, genome-wide expression analysis and pathway-based expression analysis

Software

• Quantity One ( Software )